Spectrophotometry
Chemistry ⇒ Analytical Chemistry
Spectrophotometry starts at 12 and continues till grade 12.
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A sample absorbs 75% of the incident light. What is its transmittance?
A solution has a molar absorptivity of 200 L·mol⁻¹·cm⁻¹, a path length of 2 cm, and a concentration of 0.01 mol/L. What is its absorbance?
A solution has an absorbance of 0.699. What is its transmittance as a percentage?
A solution transmits 10% of the incident light. What is its absorbance?
A student measures the absorbance of a solution at different concentrations and finds a non-linear relationship. What could be a possible reason?
A student prepares a calibration curve by measuring absorbance of standard solutions. What is the purpose of this curve?
Calculate the concentration of a solution with absorbance 0.5, path length 1 cm, and molar absorptivity 100 L·mol⁻¹·cm⁻¹.
Define transmittance in the context of spectrophotometry.
Describe how a calibration curve is constructed in spectrophotometry.
Describe the function of the detector in a spectrophotometer.
Explain why it is important to clean cuvettes before use in spectrophotometry.
Explain why it is important to use a blank solution in spectrophotometric analysis.
Explain why quartz cuvettes are used for UV measurements instead of glass cuvettes.
If a solution has an absorbance of 0.301, what is its percent transmittance?
The Beer-Lambert Law is expressed as A = εlc. What does 'ε' represent?
What is spectrophotometry?
What is the effect of increasing the path length of the cuvette on absorbance, assuming concentration and molar absorptivity remain constant?
What is the main advantage of using a double-beam spectrophotometer over a single-beam spectrophotometer?
What is the purpose of a monochromator in a spectrophotometer?
