Sterilization and Aseptic Techniques
Biology ⇒ Microorganisms and Microbiology
Sterilization and Aseptic Techniques starts at 10 and continues till grade 12.
QuestionsToday has an evolving set of questions to continuously challenge students so that their knowledge grows in Sterilization and Aseptic Techniques.
How you perform is determined by your score and the time you take.
When you play a quiz, your answers are evaluated in concept instead of actual words and definitions used.
See sample questions for grade 11
A laboratory technician needs to sterilize a solution of vitamins that are heat-labile. Which method should they use?
A researcher needs to sterilize a solution containing antibiotics that degrade at high temperatures. Which method should be used and why?
A student is preparing to inoculate a sterile agar plate with bacteria. List three aseptic techniques they should follow to prevent contamination.
Describe the difference between antiseptics and disinfectants.
Describe the difference between sterilization and disinfection.
Describe the importance of using aseptic techniques in clinical settings.
Describe the principle of autoclaving and explain why it is effective for sterilization.
Describe the role of HEPA filters in maintaining aseptic conditions in laboratories.
Explain why filtration is preferred over autoclaving for sterilizing certain solutions.
Explain why flaming the mouth of a test tube is important during aseptic transfer.
Explain why it is important to cool the inoculating loop before picking up bacteria.
Explain why it is important to label all sterilized materials with the date and method of sterilization.
Name one advantage and one disadvantage of using dry heat sterilization.
A laboratory is testing the effectiveness of a new sterilization method. After treatment, samples are incubated in nutrient broth, and turbidity is observed in some tubes. What does the presence of turbidity indicate, and what should be the next step in evaluating the sterilization method?
A researcher needs to sterilize a solution containing both heat-stable and heat-labile components. Suggest a protocol that ensures complete sterilization without damaging the heat-labile components.
Describe how the use of positive and negative controls can help validate the effectiveness of a sterilization process in a laboratory experiment.
Explain why prions are particularly challenging to eliminate during sterilization processes, and describe one method that can be used to reduce prion contamination.
Explain why the use of aseptic techniques is especially important when working with pathogenic microorganisms in a laboratory setting.
