Recombinant DNA Technology
Biology ⇒ Biotechnology and Applications
Recombinant DNA Technology starts at 9 and continues till grade 12.
QuestionsToday has an evolving set of questions to continuously challenge students so that their knowledge grows in Recombinant DNA Technology.
How you perform is determined by your score and the time you take.
When you play a quiz, your answers are evaluated in concept instead of actual words and definitions used.
See sample questions for grade 11
Describe one ethical issue related to the use of recombinant DNA technology.
Describe the difference between a genomic library and a cDNA library.
Describe the function of a multiple cloning site (MCS) in a vector.
Describe the role of Agrobacterium tumefaciens in plant genetic engineering.
Explain the difference between sticky ends and blunt ends produced by restriction enzymes.
Explain the role of selectable markers in recombinant DNA technology.
Explain the significance of the origin of replication (ori) in a cloning vector.
Explain why antibiotic resistance genes are used in plasmid vectors.
Explain why sticky ends are important in recombinant DNA technology.
Name the process by which a recombinant DNA molecule is introduced into a host cell.
A biotechnology company is developing a genetically modified bacterium to produce a human enzyme. What two features must the expression vector contain to ensure both selection and high-level expression of the enzyme?
A researcher wants to express a human gene in bacteria. List and explain two challenges that must be addressed to ensure successful expression of the gene in the bacterial host.
Describe the role of reporter genes in the identification of recombinant cells and provide one example of a commonly used reporter gene.
Explain how the use of restriction enzymes and DNA ligase enables the creation of recombinant DNA molecules. Include the importance of compatible ends in your answer.
Explain why the use of a strong promoter is important when expressing a foreign gene in a host organism using recombinant DNA technology.
