Principles of Genetic Engineering
Biology ⇒ Biotechnology and Applications
Principles of Genetic Engineering starts at 9 and continues till grade 12.
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A researcher uses a restriction enzyme to cut a plasmid and a gene of interest. What must be true about the ends of the DNA fragments for successful ligation?
A scientist wants to clone a gene into a plasmid vector. Which enzyme should be used to join the gene and the plasmid?
Describe the difference between sticky ends and blunt ends produced by restriction enzymes.
Describe the function of restriction endonucleases in genetic engineering.
Describe the importance of palindromic sequences in genetic engineering.
Describe the process of transformation in genetic engineering.
Explain the importance of the origin of replication in a plasmid vector.
Explain the principle of the polymerase chain reaction (PCR).
Explain the role of a selectable marker in genetic engineering.
Explain why antibiotic resistance genes are often used as selectable markers in plasmid vectors.
Explain why Taq polymerase is preferred in PCR.
