Principles of Genetic Engineering
Biology ⇒ Biotechnology and Applications
Principles of Genetic Engineering starts at 9 and continues till grade 12.
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Describe the function of restriction enzymes in genetic engineering.
Describe the process of transformation in genetic engineering.
Explain the role of selectable markers in genetic engineering.
Explain why genetic engineering is important in medicine.
Explain why plasmids are commonly used as vectors in genetic engineering.
What is a plasmid?
What is meant by the term 'recombinant DNA'?
What is the function of a selectable marker in a vector?
What is the main purpose of using a vector in genetic engineering?
What is the role of host cells in genetic engineering?
What is the significance of sticky ends in genetic engineering?
Which enzyme is used to cut DNA at specific sequences?
A scientist wants to insert a human gene into a bacterial plasmid. The plasmid has two restriction sites: EcoRI and HindIII. The human gene is flanked by EcoRI sites. Which restriction enzyme(s) should the scientist use to ensure successful insertion, and why?
A student claims that genetic engineering can only be used to add genes to an organism. Critically evaluate this statement.
Describe one method by which scientists can confirm that a foreign gene has been successfully inserted and expressed in a host organism.
Explain how the use of antibiotic resistance genes as selectable markers in genetic engineering can pose environmental and health risks. Suggest an alternative strategy for selection and justify your answer.
Explain why it is important to use the same restriction enzyme to cut both the vector DNA and the foreign DNA fragment in genetic engineering.
