Polymerase Chain Reaction
Biology ⇒ Biotechnology and Applications
Polymerase Chain Reaction starts at 11 and continues till grade 12.
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See sample questions for grade 12
A PCR reaction contains 1,000 template DNA molecules. After 10 cycles, how many DNA molecules will there be, assuming 100% efficiency?
A PCR reaction is set up with 30 cycles. Ideally, how many copies of the target DNA will be produced from a single template molecule?
A student sets up a PCR reaction but forgets to add primers. What will happen?
Describe one application of PCR in the field of medicine.
Describe one way to prevent contamination in PCR experiments.
Describe the difference between standard PCR and real-time PCR (qPCR).
Describe the role of dNTPs in a PCR reaction.
Explain the importance of magnesium ions (Mg2+) in a PCR reaction.
Explain the principle of nested PCR and its advantage.
Explain why contamination is a significant concern in PCR experiments.
Explain why Taq polymerase is preferred over other DNA polymerases in PCR.
In a PCR reaction, what is the function of primers?
List the three main steps of a standard PCR cycle in the correct order.
What is the main advantage of using PCR over traditional cloning methods for DNA amplification?
Who developed the Polymerase Chain Reaction (PCR) technique?
A laboratory technician accidentally uses a DNA polymerase that is not thermostable in a PCR reaction. Predict and explain the outcome of the PCR.
A researcher is attempting to amplify a DNA fragment with a high GC content using PCR. Explain two modifications to the standard PCR protocol that could improve the amplification of such GC-rich templates.
A scientist performs a PCR with the following cycle times: denaturation for 30 seconds at 95°C, annealing for 30 seconds at 60°C, and extension for 1 minute at 72°C. If the target DNA fragment is 1.5 kb, is the extension time sufficient? Justify your answer.
Describe how PCR can be used to introduce a restriction enzyme site into a DNA fragment.
Explain the concept of 'hot-start PCR' and describe one advantage it offers over conventional PCR.
