Polymerase Chain Reaction
Biology ⇒ Biotechnology and Applications
Polymerase Chain Reaction starts at 11 and continues till grade 12.
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See sample questions for grade 11
Describe one limitation of PCR.
Describe the difference between conventional PCR and real-time (quantitative) PCR.
Describe the importance of the denaturation step in PCR.
Explain the significance of the annealing temperature in PCR.
Explain why PCR is considered a revolutionary technique in molecular biology.
Explain why Taq polymerase is used in PCR instead of human DNA polymerase.
If a PCR reaction is run for 10 cycles, starting with 2 DNA molecules, how many DNA molecules will be present at the end?
In a standard PCR, if you start with 1 DNA molecule, how many copies will you have after 5 cycles?
List the three main steps of a PCR cycle in order.
Name one application of PCR in forensic science.
The function of primers in PCR is to ________.
What does PCR stand for in biotechnology?
What is the function of a thermal cycler in PCR?
What is the main purpose of the extension step in PCR?
What is the role of magnesium ions (Mg²⁺) in PCR?
What would happen if the primers used in PCR are not specific to the target DNA sequence?
Which enzyme is essential for the synthesis of new DNA strands during PCR?
A researcher wants to amplify a DNA fragment that is 15,000 base pairs long. Is standard PCR suitable for this purpose? Justify your answer.
A student sets up a PCR reaction but accidentally omits dNTPs. Predict the outcome and explain why.
Calculate the theoretical number of DNA copies produced after 8 cycles of PCR, starting with a single DNA molecule. Show your working.
